Primer

Part:BBa_K5427019:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV Keratin Pet22B+


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 24
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 24
    Illegal NotI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 24
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reverse primer for amplification of keratin31 insert in pET22b+.


Source

Not applicable.


References